AFAIK these are single ended SCSI devices. So you need a SCSI card that has a 50-way, SE (single-ended) SCSI bus.
What card you choose will depend on what interface bus the Windows 2000 machine has. If it's PCI, then typically you'd use something like an Adaptec AHA2940 card, which you should be able to get super-cheap on eBay.
You'll need a SCSI 50 way cable, (high density 50-way to probably low density 50-way Centronics, depending on what the connector is on the DLT box) and a 50-way SE (preferably active) terminator.